Cheap IBI Scientific IB47281 Blood/Tissue/Cultured Cell gMax Mini Genomic DNA Kit for 100 Preparations

Cheap IBI Scientific IB47281 Blood/Tissue/Cultured Cell gMax Mini Genomic DNA Kit for 100 Preparations  review

Product Description

The gMax mini kit provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood (fresh/frozen blood), tissue, buccal swab and amniotic fluid. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column (1). Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt elution buffer or TE. The entire procedure can be completed in less than 30 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 microgram from 200 microliter of whole human blood. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions. The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 microliter of whole human blood. The purified DNA (4-6 microgram with an A260/A280 ratio of 1.6 - 1.8) is quantified with a spectrophotometer and checked by electrophoresis. Caution: GSB Buffer contains guanidine hydrochloride which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, and protective goggles. Spin column format. Yield: up to 50 microgram. Operation time: 30 minutes or less. Elution volume: 50-200 microliter.

Cheap IBI Scientific IB47281 Blood/Tissue/Cultured Cell gMax Mini Genomic DNA Kit for 100 Preparations  features

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Product Details

• Size: 100 Preps
• Brand: IBI
• Model: IB47281
• Number of items: 1

Features

• Kit contains 50 Mini GD columns, (100) 2mL collection tubes, 60mL GST buffer, 60mL GSB buffer, 45mL W1 buffer, 25mL wash buffer (100mL add Ethanol)
• It also contains 30mL elution buffer and 11mg-x2 Proteinase K (1.1mL add ddH2O)
• For research use only, Extract genomic DNA from blood, tissue, buccal swab, and amniotic fluid
• Sample size: 50-200 microliter of whole blood (fresh/frozen blood), up to 25mg of tissue, up to 25mg of paraffin-embedded tissue, buccal swab, up to 15 ml of amniotic fluid
• Operation: centrifuge

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